3D Human Blood Brain Barrier

Description

Product Name 3D Human Blood Brain
Barrier
Catalog Number EP010
Product Format 6 , 12, and 24 well
Storage Temperature -80℃.
Shipping Temperature -80℃.
CNS pharmaceutics market: Only a few central nervous system (CNS) disorders, such as
depression, epilepsy, chronic pain, and affective disorders, respond to clinical treatments
by the 2% of small-molecule drugs that we have; on the other hand, many more serious
CNS disorders cannot be effectively treated by these small therapeutic molecules including
Alzheimer disease, Huntington disease, stroke, brain cancer, brain and spinal cord injury,
HIV infection of the brain, therefore we want to help change that by creating our 3DHuman
BBB model. The blood brain barrier (BBB) specifically regulates molecular and cellular
flux between the blood and the nervous tissue. We develop and characterize a highly
reproducible Human in vitro model of the BBB using co-cultures of primary Human brain
endothelial cells (HBEC), Human brain pericytes, and Human brain astrocytes to study
receptors involved in transcytosis across the endothelial cell monolayer. Many drugs
developed to treat Central Nervous System (CNS) disorders are unable to reach the brain
parenchyma in therapeutically relevant concentrations. The BBB protects brain nervous
tissue from the fluctuation of plasma composition, from pathogenic agents, and maintains
homeostasis of the brain parenchyma by restricting non-specific flux of ions, peptides,
proteins and even cells into and out the brain.Astrocytes and brain pericytes help to develop and maintain specific BBB characteristics in
brain capillary endothelial cells. Co-culture of the three cell types in our 3D Human BBB
model led to the enhancement of barrier properties; an increase in expressions of tight
junction proteins of occludin, claudin-5 and ZO-1 and continuous localizations of ZO-1 and
claudin-5.Our model mimic transport properties of the BBB due to the formation of tight junctions,
higher expression of specific carriers, or great cell viability. We developed a 3D in vitro
model of the BBB by culturing brain endothelial cells with pericytes and astrocytes layered in an insert. This model improves endothelial cell polarization and enhance the
formation of tight junctions, provide better endothelial cell-to-cell contact that is
important for barrier development, and prevent the dilution of secreted
neurotrophic factors, and these conditions collectively led to the development of an in
vitro model that can truly mimic the BBB.
Advantages:
1) Cells used in the 3D model are all human cells; results obtained are more relevant to
human situations rather than those data from animal models, i.e. CAM et al.
2) The whole process can be monitored (from cell inoculation to the end of
experiment), therefore, more crucial information can be acquired at multiple time
points from a single experiment.
3) No need to perform post-experimental staining for endothelial markers, this is
particularly important, if those markers are changed in experimental conditions
involved in the studies.
The 3D Human BBB Model contains all the materials necessary to perform multiple
angiogenesis assays in 6, 12, or 24 well formats.
The 3D Human BBB model can be use, but not limited to:
• Drug BBB permeability assay
• Research on BBB physiology
• Cell-cell interactions
• Transport pathway modulations
• Research on BBB toxicology
• Brain endothelial toxicity assays
• Research on BBB pathology
• Disease modeling Microvessels
• Transport and permeability studies from ions to macromolecules: effect of
physiological or pathogenetic factors
• Paracellular barrier and cell polarity studies: TJ protein expression, distribution,
polarized distribution of transport proteins, receptors, enzymes etc
• Studies on endo- and transcytosis, receptor-ligand interactions • Drug transport, drug effect on permeability, localization of receptors, polarity of
drug responses
• Co-culture studies: cell-cell and cell-matrix interactions
• Microbial pathogenesis: virus, bacteria, parasite attachment, invasion and
penetration
• Compounds screening neuroimmune targets

International:

May vary, we will contact you!