Anti – Bcl – 2 Antibody (Monoclonal)

Description

Product Name Anti-Bcl-2 antibody (monoclonal)
Description Mouse IgG monoclonal antibody for Bcl-2, B-cell CLL/lymphoma 2
(BCL2) detection. Tested with WB, IHC-P, IHC-F, ICC in Human.
No cross reactivity with other proteins.
Host Mouse
Target Species Human
Application WB,HC-P, IHC-F, ICC,
Recommended
Detection
Systems
We recommends Enhanced Chemiluminescent Kit with anti-Mouse
IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse
IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and
ICC.
*Blocking peptide can be purchased. Contact us for more
information
Immunogen Recombinant full-length BCL2
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog Number Ab1004

Background:
Immunoreactive BCL2 protein in the neoplastic cells of almost all follicular lymphomas
whereas no BCL2 protein was detected in follicles affected by nonneoplastic processes
or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18)
translocation expressed detectable levels of BCL2 protein, regardless of whether the
breakpoint was located in or at a distance from the BCL2 gene. Overexpression of
BCL2 blocks the apoptotic death of a pro-B-lymphocyte cell line.
Product Properties:
Clonality Monoclonal
Clone Number Bcl-2-100
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg
NaN3 as preservative.
*carrier free antibody available upon request.
Reconstitution Add 1ml of 1XPBS buffer (catalog # 1XPBS-001) will yield a
concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It
can also be aliquoted and stored frozen at -20˚C for a longer time.
Avoid repeated freezing and thawing.
Purification Ascites
Application Details:
Application Concentration* Tested
Species
Predicted
Species
Antigen
Retrieval
Western blot 1-2μg/ml Human –
Immunohistochemistry(Paraffinembedded Section)
0.4-1μg/ml Human – By Heat
Immunohistochemistry(Frozen
Section)
0.4-1μg/ml Human –
Immunocytochemistry 1μg/ml Human

Protein Target:
Gene Name BCL2
Protein Name Apoptosis regulator Bcl-2
Gene Full Name B-cell CLL/lymphoma 2
Protein Function Suppresses apoptosis in a variety of cell systems including
factor-dependent lymphohematopoietic and neural cells.
Regulates cell death by controlling the mitochondrial membrane
permeability. Appears to function in a feedback loop system
with caspases. Inhibits caspase activity either by preventing the
release of cytochrome c from the mitochondria and/or by
binding to the apoptosis-activating factor (APAF-1).
Tissue Specificity Expressed in a variety of tissues.
Sequence
Similarities
Belongs to the Bcl-2 family.
Subcellular
Localization
Mitochondrion outer membrane.
Uniprot ID Human(P10415), Mouse(P10417), Rat(P49950)
Alternative Names Apoptosis regulator Bcl-2|BCL2|ApoptosisregulatorBcl2|Apoptosis regulator Bcl2|ApoptosisregulatorBcl2

International:

May vary, we will contact you!