Anti – c – Myc Antibody (Monoclonal, 9E10)

Description

Product Name Anti-c-Myc Antibody (Monoclonal, 9E10)
Description Mouse IgG monoclonal antibody for c-Myc, v-myc myelocytomatosis viral
oncogene homolog (avian) (MYC) detection. Tested with WB, IHC-P, ICC in
Human. No cross reactivity with other proteins.
Host Mouse
Target Species Human
Application IHC-P, ICC, WB
Recommended
Detection Systems
We recommend Enhanced Chemiluminescent Kit with anti-Mouse IgG
(EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super
Vision Assay Kit (SV0001-1) for IHC(P) and ICC.
Immunogen Synthetic peptide corresponding to residues 408-439 of the human p62cMyc protein.
Specificity No cross reactivity with other proteins
Pack Size 100μg/vial
Catalog # Ab1028
Properties
Clonality Monoclonal
Clone Number 9E10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as
preservative.
*carrier free antibody available upon request.

Reconstitution Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also
be aliquoted and stored frozen at -20˚C for a longer time. Avoid repeated
freezing and thawing.
Purification Ascites
Background for Myc proto-oncogene protein
C-Myc is an oncogene that functions both in the stimulation of cell proliferation and in apoptosis. c-Myc
elicits its oncogenic activity by causing immortalization, and to a lesser extent the transformation of cells,
in addition to several other mechanisms. The c-MYC proto-oncogene encodes a transcription factor that
is critical for cell growth and proliferation. It is one of the genes frequently altered in cancer cells in which
it exhibits constitutive activity. Downregulation of c-Myc is critical for 2-Methoxyestradiol(2ME2)-induced
oxidative stress and apoptosis in AML cells. And its up-regulation is important for promoting lymphocyte
cell division, and demonstrating that GFP-c-Myc expression is a marker of proliferating lymphocytes in
vivo.
Application Details
Application Concentration* Tested
Species
Predicted
Species
Antigen
Retrieval
Western blot 4μg/ml Human –
Immunohistochemistry(Paraffinembedded Section)
8μg/ml Human – By Heat
Immunocytochemistry 1μg/ml Human –
Price: $150
Protein Target Info (Source: Uniprot.org)
Gene Name MYC
Protein Name Myc proto-oncogene protein
Gene Full Name v-myc myelocytomatosis viral oncogene homolog(avian)

Protein Function Transcription factor that binds DNA in a non-specific manner, yet also
specifically recognizes the core sequence 5′- CAC[GA]TG-3′. Activates the
transcription of growth-related genes.
Sequence Similarities Contains 1 bHLH (basic helix-loop-helix) domain.
Subcellular
Localization
Nucleus, nucleoplasm.
Uniprot ID Human(P01106), Mouse(P01108), Rat(P09416)
Alternative Names MYC|Myc proto-oncogene protein|Mycproto-oncogeneprotein|Myc
protooncogene protein|Mycprotooncogeneprotein

International:

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