Product Information

Product Description

Dulbecco's Modified Eagle Medium (DMEM), is a modified medium based on MEM, is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs, and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.

The mammalian target of rapamycin (mTOR) complex 1 (mTORC1) plays an important role in cell growth and metabolism. In recent years, Leucine (Leu) activation of mTORC1 has become more of a research hotspot (Appuhamy et al., 2012; Averous et al., 2016; Dai et al., 2015; Gu et al., 2022; Kim et al., 2017; Son et al., 2019; Takayama et al., 2018). Therefore, there is a need for leucine-deficient cell culture medium for basic research at the cellular levels.

This product is a modified medium based on DMEM high glucose medium with L-leucine removed and sterilized by 0.1 filter membrane, pH 7.0-7.4. Please check the official website for specific ingredients and concentrations.

Storage and Shipping Conditions

Ship at room temperature; Store away from light at 2-8℃, valid for 12 months.

Note

1. This product has been filtered and sterilized. Attention should be paid to aseptic operation to avoid contamination.

2. For best results, do not freeze and thaw repeatedly.

3. For your safety and health, please wear safety glasses, gloves or protective clothing.

References

Appuhamy, J.A.D.R.N., Knoebel, N.A., Nayananjalie, W.A.D., Escobar, J., and Hanigan, M.D. (2012). Isoleucine and Leucine Independently Regulate mTOR Signaling and Protein Synthesis in MAC-T Cells and Bovine Mammary Tissue Slices. The Journal of Nutrition 142, 484-491.

Averous, J., Lambert-Langlais, S., Mesclon, F., Carraro, V., Parry, L., Jousse, C., Bruhat, A., Maurin, A.-C., Pierre. P., Proud, C.G., et al. (2016). GCN2 contributes to mTORC1 inhibition by leucine deprivation through an ATF4 independent mechanism.Scientific Reports 6, 27698.

Dai, J.M., Yu, M.X., Shen, Z.Y., Guo, C.Y., Zhuang, S.Q., and Qiu, X.S. (2015). Leucine Promotes Proliferation and Differentiation of Primary Preterm Rat Satellite Cells in Part through mTORC1 Signaling Pathway.Nutrients 7, 3387-3400.

Gu, X., Jouandin, P., Lalgudi, P.V., Binari, R., Valenstein, M.L., Reid, M.A., Allen, A.E., Kamitaki, N., Locasale, J.W., Perrimon, N., et al. (2022). Sestrin mediates detection of and adaptation to low-leucine diets in Drosophila.Nature 608, 209-216.

Kim, J. H., Lee, C., Lee, M., Wang, H., Kim, K., Park, S.J., Yoon, I., Jang, J., Zhao, H., Kim, H.K., et al. (2017). Control of leucine-dependent mTORC1 pathway through chemical intervention of leucyl-tRNA synthetase and RagD interaction.Nature communications8, 732.

Son, S.M., Park, S.J., Lee, H., Siddiqi, F., Lee, J.E., Menzies, F.M., and Rubinsztein, D.C (2019). Leucine Signals to mTORC1 via Its Metabolite Acetyl-Coenzyme A.Cell Metabolism 29, 192-201. e197.

Takayama, K., Muto, A., and Kikuchi, Y. (2018). Leucine/glutamine and v-ATPase/lysosomal acidification via mTORC1 activation are required for position-dependent regeneration.Scientific Reports 8, 8278.

For Research Use Only! Not for use in diagnostic procedures!

Version: 2.1